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TOP Science is a Biochemical technology Co.LTD, And we provide a full range of CRO services that cover the entire drug development pipeline, including contract research for compound synthesis, biochemical and cellular analysis,and marketing research.

Structure-Function Studies of the Neisseria gonorrhoeae

The major outer membrane porin (PorB) expressed by Neisseria gonorrhoeae plays multiple roles during infection, in addition to its function as an outer membrane pore. We have generated a panel of mutants of N. gonorrhoeae strain FA1090 expressing a variety of mutant porB genes that all function as porins. We identified multiple regions of porin that are invo

2013-12-12 11:18:09 | [Read more...]

Angelo Miguel Figueiredo, Sara B.-M. Whittaker, Stuart

The diversity of possible conformational states makes it challenging to obtain a structural description of how proteins fold. Clusters of hydrophobic residues in unfolded states of a protein help determine interatomic contacts in the early stages of folding. Here, the authors describe dynamics of an Im7 variant designed to increase the helical propensity of

2013-12-4 9:37:12 | [Read more...]

Structure-Function Studies of the Neisseria gonorrhoeae

The major outer membrane porin (PorB) expressed by Neisseria gonorrhoeae plays multiple roles during infection, in addition to its function as an outer membrane pore. We have generated a panel of mutants of N. gonorrhoeae strain FA1090 expressing a variety of mutant porB genes that all function as porins. We identified multiple regions of porin that are invo

2013-11-28 10:12:29 | [Read more...]

Analysis of off-target effects of CRISPR/Cas-derived RN

RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enzymes cleave chromosomal DNA, whose sequence is complementary

2013-11-27 11:53:30 | [Read more...]

The common problems and solutions in ELISA

We have a solution for all your ELISA problems, from a poor standard curve to a large coefficient of variation (CV). 1. View below our troubleshooting tips including: 1) Poor standard curve 2) Low signal 3) Large coefficient of variation (CV) 4) High background 5) Low sensitivity 6) General applicable tips 2.Genera

2012-9-4 23:18:33 | [Read more...]

Mag-Bind® Soil DNA Kit Protoco

Mag-Bind® Soil DNA Kit Protoco

2012-9-4 23:17:14 | [Read more...]

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